317-622-8970Search all companies →Largest Healthcare & Life Science Companies in Minnesota (2026)
Factur's directory lists 81,197 healthcare & life science companies in Minnesota. These are the 10 largest of them by annual revenue, led by Mayo Clinic ($11.2B), Prime Therapeutics LLC ($8.8B) and Sevita ($5.9B).
Size mix: of the 81,194 with a known headcount, 97.9% have 1-50 employees, 1.7% 51-200, 233 companies 201-1000, 36 companies 1001-5000 and 24 companies 5001+. 107 employ more than 500 people.
The 10 largest healthcare & life science companies in Minnesota by annual revenue
| # | Company | City | Employees | Annual revenue |
|---|---|---|---|---|
| 1 | Mayo Clinic | Rochester | 76,000 | $11.2B |
| 2 | Prime Therapeutics LLC | Eagan | 7,000 | $8.8B |
| 3 | Sevita | Edina | 40,000 | $5.9B |
| 4 | International Dairy Queen Inc | Bloomington | 10,000 | $5B |
| 5 | Fairview Health Services | Minneapolis | 32,637 | $4.4B |
| 6 | Allina Health | Minneapolis | 27,000 | $4B |
| 7 | HealthPartners | Bloomington | 26,000 | $3.8B |
| 8 | Life Time Inc | Chanhassen | 43,000 | $3B |
| 9 | Park Nicollet Methodist Hospital | Minneapolis | 3,500 | $2.8B |
| 10 | Essentia Health | Duluth | 15,456 | $2.8B |
How this list is ranked
Companies are ranked by annual revenue (USD) as recorded on each company's profile in the Factur directory, highest first; companies with the same revenue are ordered by employee count. Only companies with a positive revenue figure on file are ranked, so a large private company with no reported revenue can be missing. The list covers companies whose record places them in Minnesota and whose Factur industry is Healthcare & Life Science. Figures are a snapshot of the directory, refreshed when the rankings are regenerated; open a profile for its current data.