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Largest Healthcare & Life Science Companies in Wisconsin (2026)

Factur's directory lists 67,420 healthcare & life science companies in Wisconsin. These are the 10 largest of them by annual revenue, led by Milwaukee Bucks ($4B), University of Wisconsin Hospitals and Clinics Authority ($3.5B) and ThedaCare ($3.2B).

Size mix: of the 67,420 with a known headcount, 99% have 1-50 employees, 487 companies 51-200, 173 companies 201-1000, 24 companies 1001-5000 and 15 companies 5001+. 76 employ more than 500 people.

The 10 largest healthcare & life science companies in Wisconsin by annual revenue

#CompanyCityEmployeesAnnual revenue
1Milwaukee BucksMilwaukee680$4B
2University of Wisconsin Hospitals and Clinics AuthorityMadison24,000$3.5B
3ThedaCareAppleton22,000$3.2B
4University of Wisconsin-MadisonMadison26,755$3B
5Marshfield Clinic Health SystemMarshfield12,000$2.9B
6Froedtert & The Medical College of WisconsinMilwaukee17,727$2.6B
7Emplify HealthGreen Bay15,000$2.2B
8Aspirus IncWausau14,000$2.1B
9Culver Franchising SystemPrairie Du Sac25,000$1.8B
10Children's WisconsinMilwaukee6,200$1.7B

How this list is ranked

Companies are ranked by annual revenue (USD) as recorded on each company's profile in the Factur directory, highest first; companies with the same revenue are ordered by employee count. Only companies with a positive revenue figure on file are ranked, so a large private company with no reported revenue can be missing. The list covers companies whose record places them in Wisconsin and whose Factur industry is Healthcare & Life Science. Figures are a snapshot of the directory, refreshed when the rankings are regenerated; open a profile for its current data.